Journal article
Opsonising Antibodies to P. falciparum Merozoites Associated with Immunity to Clinical Malaria
DL Hill, EM Eriksson, CSN Li Wai Suen, CY Chiu, V Ryg-Cornejo, LJ Robinson, PM Siba, I Mueller, DS Hansen, L Schofield
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2013
Abstract
Naturally acquired humoral immunity to the malarial parasite Plasmodium falciparum can protect against disease, although the precise mechanisms remain unclear. Although antibody levels can be measured by ELISA, few studies have investigated functional antibody assays in relation to clinical outcomes. In this study we applied a recently developed functional assay of antibody-mediated opsonisation of merozoites, to plasma samples from a longitudinal cohort study conducted in a malaria endemic region of Papua New Guinea (PNG). Phagocytic activity was quantified by flow cytometry using a standardized and high-throughput protocol, and was subsequently evaluated for association with protection fro..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work is supported by the Cancer Research UK and EPSRC Cancer Imaging Centre in association with the MRC and Department of Health (England) (refs C1060/A10334). The authors acknowledge NHS Funding to the NIHR Biomedical Research Centre. MFM and MBB acknowledge funding from Cancer Research UK (grant C1060/A6916), Marie Curie Action: Early Stage Training (grant MEST-CT-2005 020718), and EPSRC platform (grant EP/H046526/1). MOL is an NIHR Senior Investigator. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.